Latest Products http://www.umbrellalifescience.co.in Latest Products Mon, 20 Sep 2021 02:47:38 +0530 en-us http://www.umbrellalifescience.co.in DNA Isolation Kit http://www.umbrellalifescience.co.in/dna-isolation-kit.htm http://www.umbrellalifescience.co.in/dna-isolation-kit.htm Mon, 17 Jun 2019 00:00:00 +0530 Plant DNA Isolation Kit http://www.umbrellalifescience.co.in/plant-dna-isolation-kit.htm http://www.umbrellalifescience.co.in/plant-dna-isolation-kit.htm Mon, 17 Jun 2019 00:00:00 +0530 Features The Plant DNA Kit provides a fast and simple method to isolating DNA from Plant tissues. In the process, sample crushed with liquid nitrogen and lysed further with Lysis buffer . In the presence of binding buffer with chaotropic salt, the genomic DNA in the lysate binds to the silica column. The DNA is bound to the column while proteins and other impurities are removed by wash buffer.   Advantages & Features: Highly efficient : yields up to 5˞-40 μg total DNA from young leaves. Pure genomic DNA : ready-to-use in all Molecular Biology applications. Really easy and fast procedure : it takes 40 minutes to results with minimal handling steps. Versatile : high quality DNA obtained from several types of plants.  Mini format.   Applications : Purified DNA suitable for all common Molecular Biology applications, such as: RT-PCR. Southern blotting. RFLP. Blood and Tissue DNA Isolation Kit http://www.umbrellalifescience.co.in/blood-and-tissue-dna-isolation-kit.htm http://www.umbrellalifescience.co.in/blood-and-tissue-dna-isolation-kit.htm Mon, 17 Jun 2019 00:00:00 +0530 Features Blood and Cell Culture DNA kit is designed for rapid and efficient purification of genomic DNA from blood, body fluids (amniotic fluid, saliva, lymph), and mammalian cells. Sample sources include fresh and frozen whole blood treated with common anticoagulants such as citrate, EDTA and heparin. In addition plasma, serum, buffy coat, bone marrow, lymphocytes, platelets, and body fluid samples can also be used.   Advantages & Features : Fast protocol : results in 25 minutes. High-quality genomic DNA purification from various sources. Reproducible extraction of high molecular weight genomic DNA purified. Safe and convenient : avoids phenol/chloroform extraction. Efficient : 10-40 μg of genomic DNA from a 200 μl blood sample. Pure genomic DNA : ready-to-use in all Molecular Biology applications.    Applications : Purified DNA suitable for all common Molecular Biology applications, such as: PCR Cloning DNA sequencing Southern blot analysis    Quality Control : Tested on a lot-to-lot basis by isolating total DNA from 200 μl of whole Human blood. DNA purified is analysed by: Spectrophotometer: Ratio 260/280 (1.8-2.0). Agarose gel electrophoresis. Bacterial DNA Isolation Kit http://www.umbrellalifescience.co.in/bacterial-dna-isolation-kit.htm http://www.umbrellalifescience.co.in/bacterial-dna-isolation-kit.htm Mon, 17 Jun 2019 00:00:00 +0530 Features Bacterial DNA Isolation Kit provides an accurate, easy-to use and rapid method to isolate high quality DNA from both Gram negative and Gram positive bacteria. The kit uses Fast-Spin column technology based in the ability to bind silica in the presence of high concentrations of chaotropic salts as guanidinium thiocyanate. The extraction process uses comfortable Mini Spin Columns and includes an initial cell-wall lysis step with the appropriate enzyme to ensure efficient cell lysis and DNA release from the cell.   Advantages & Features : Highly efficient : yields up to 24 μg. Really fast and easy procedure : it takes 45 minutes to get results with minimal handling steps Safe : avoids phenol/chloroform extraction. Convenient : ideal for bacterial DNA isolation from cell pellets after culturing    Applications : Purified DNA suitable for all common Molecular Biology applications, such as: PCR Cloning DNA sequencing Southern blot analysis    Quality Control : Tested on a lot-to-lot basis by isolating total DNA from E. coli. The quantity and quality of purified DNA attend to: Ratio 260/280 (1.8-2.0). Agarose gel electrophoresis. Plasmid DNA Isolation Kit http://www.umbrellalifescience.co.in/plasmid-dna-isolation-kit.htm http://www.umbrellalifescience.co.in/plasmid-dna-isolation-kit.htm Mon, 17 Jun 2019 00:00:00 +0530 Features Plasmid Isolation kit offers a reliable, convenient and rapid method for routinely isolation of high quality plasmid preparations in mini format. The kit uses Fast-Spin column technology based in DNA ability to bind silica in the presence of high concentrations of chaotropic salts.   Advantages & Features: Highly efficient : yields up to 24 μg. Pure plasmid DNA : ready-to-use in all Molecular Biology applications. Really fast and easy procedure : it takes 56 minutes to results with minimal handling steps. Safe : avoids phenol/chloroform extraction. Convenient : ideal for routinely isolation of high quality plasmid preparations    Applications: Purified DNA suitable for all common Molecular Biology applications, such as: Digestion with restriction enzymes. Automated sequencing. PCR template. Bacterial transformation. Transfection.    Quality control: Tested for the isolation of any plasmid DNA from transformed E.coli. The quality of purified DNA is analysed by: Ratio 260/280 (1.8-2.0). Agarose gel electrophoresis. Insect DNA Isolation Kit http://www.umbrellalifescience.co.in/insect-dna-isolation-kit.htm http://www.umbrellalifescience.co.in/insect-dna-isolation-kit.htm Mon, 17 Jun 2019 00:00:00 +0530 Features Insect DNA Kit is designed for efficient recovery of genomic DNA from insects, arthropods, roundworms, flatworms and etc. The method is suitable for fresh as well as frozen insect samples. The kit uses Fast-Spin column technology based in DNA ability to bind silica in the presence of high concentrations of chaotropic salts .   Advantages & Features : Highly efficient : yields up to 24 μg. Pure insect DNA : ready-to-use in all Molecular Biology applications. Really fast and easy procedure : it takes 50 minutes to results with minimal handling steps. Safe : avoids phenol/chloroform extraction.    Applications: Purified DNA suitable for all common Molecular Biology applications, such as: Digestion with restriction enzymes. Automated sequencing. PCR template.    Quality control : Tested for the isolation of any DNA from drosophila melanogaster. The quality of purified DNA is analysed by: Ratio 260/280 (1.8-2.0). Agarose gel electrophoresis. Sperm DNA Isolation kit http://www.umbrellalifescience.co.in/sperm-dna-isolation-kit.htm http://www.umbrellalifescience.co.in/sperm-dna-isolation-kit.htm Mon, 17 Jun 2019 00:00:00 +0530 Features Sperm DNA kit provides a fast and easy method for isolating DNA from semen samples. The system combines the reversible nucleic acid-binding properties of the matrix with the speed and versatility of Fast-Spin column technology to yield high quality of DNA with the OD260/OD280 ratio of 1.8-2.0. Purified DNA is suitable for PCR, restriction digestion, and hybridization techniques.   Advantages & Features : Efficient : 5-10μg of genomic DNA from a 200 μl Semen. Fast : results in less of 25 minutes. Safe : no phenol-chloroform extraction. Ready-to-use : genomic DNA, in all Molecular Biology applications.    Applications : Purified DNA suitable for all common Molecular Biology applications, such as: Digestion with restriction enzymes. Automated sequencing. PCR template.    Quality control : The quality of purified DNA is analysed by: Ratio 260/280 (1.8-2.0). Agarose gel electrophoresis. Milk DNA Isolation Kit http://www.umbrellalifescience.co.in/milk-dna-isolation-kit.htm http://www.umbrellalifescience.co.in/milk-dna-isolation-kit.htm Mon, 17 Jun 2019 00:00:00 +0530 Features This kit provides a rapid spin column method for the isolation and purification of genomic DNA from both Gram-negative and Gram-positive bacteria in milk samples. Genomic DNA is isolated in 30 minutes, free of inhibitors and ready for any number of downstream applications including PCR, qPCR and Southern Blot analysis, sequencing and more.   Advantages & Features : Efficient : 5-10 μg of genomic DNA from a 200 μl milk. Fast : results in less of 30 minutes. Safe : no phenol-chloroform extraction. Ready-to-use : genomic DNA, in all Molecular Biology applications.    Applications : Purified DNA suitable for all common Molecular Biology applications, such as: Digestion with restriction enzymes. Automated sequencing. PCR template.    Quality control : The quality of purified DNA is analysed by: Ratio 260/280 (1.8-2.0). Agarose gel electrophoresis. Yeast & Fungal DNA Isolation Kit http://www.umbrellalifescience.co.in/yeast-fungal-dna-isolation-kit.htm http://www.umbrellalifescience.co.in/yeast-fungal-dna-isolation-kit.htm Mon, 17 Jun 2019 00:00:00 +0530 Features Yeast & Fungal DNA Kit provides a fast and simple method to isolating gDNA from Fungal samples. In the process, sample crushed with liquid nitrogen and lysed further with Lysis buffer . In the presence of binding buffer with chaotropic salt, the genomic DNA in the lysate binds to the silica column. The DNA is bound to the column while proteins and other impurities are removed by wash buffer.   Advantages & Features: Proven performance : isolating DNA from yeast and other cultured fungus. Safe : no phenol-chloroform extraction. Pure and high quality genomic. DNA : ready-to-use in all Molecular Biology applications. Easy and fast protocol : results in 45 minutes.    Applications: Purified DNA suitable for all common Molecular Biology applications, such as: Digestion with restriction enzymes. Automated sequencing. PCR template. Southern Blots.    Quality Control : Tested on a lot-to-lot basis by isolating total DNA from 50 mg dry fungus. DNA purified is analysed by: Spectrophotometer: Ratio 260/280 (1.8-2.0). Agarose gel electrophoresis. Saliva DNA Isolation Kit http://www.umbrellalifescience.co.in/saliva-dna-isolation-kit.htm http://www.umbrellalifescience.co.in/saliva-dna-isolation-kit.htm Mon, 17 Jun 2019 00:00:00 +0530 Features Saliva Genomic DNA Extraction kit provides an efficient, consistent and rapid method for DNA extraction from buccal saliva. The procedure includes sample collection, lysis, protein removing, DNA precipitation, washing and hydration.   Advantages & Features : Convenient : ideal for routine. Safe : avoids phenol/chloroform extraction. Highly efficient : 10 μg of genomic DNA from saliva. Pure genomic DNA : ready-to-use in all Molecular Biology applications. Time-saving protocol : reduces sample collection distress and blood sample handling.    Applications : Purification of genomic DNA from buccal saliva of different origins (Human or animal).    Quality control : Analysed by saliva DNA genomic extraction. DNA purified is analysed by: Ratio 260/280 (1.8-2.0). Agarose gel electrophoresis. Tissue DNA Isolation Kit http://www.umbrellalifescience.co.in/tissue-dna-isolation-kit.htm http://www.umbrellalifescience.co.in/tissue-dna-isolation-kit.htm Mon, 17 Jun 2019 00:00:00 +0530 Features Tissue Genomic DNA Purification Kit offers a highly efficient, rapid and convenient method for purification of total DNA from a variety of tissues. The kit is based in DNA ability to bind silica in the presence of high concentrations of chaotropic salts.   Advantages & Features : Highly efficient : yields up to 50 μg, depends on type of sample. Pure genomic DNA : ready-to-use in all Molecular Biology applications. Easy and fast procedure : it takes 54 minutes for purification of total DNA from a variety of tissues. Mini format.    Applications : Purified DNA suitable for all common Molecular Biology applications, such as: RT-PCR. Southern blotting. RFLP.    Quality control : Total DNA is isolated from a 30 mg thorax muscle tissue sample. Purified DNA is quantified using a spectrophotometer with a typical yield of more than 10 μg of genomic DNA and an A260nm/A280nm ratio of 1.8-2.0. Quality is further checked by agarose gel electrophoresis. Soil DNA Isolation Kit http://www.umbrellalifescience.co.in/soil-dna-isolation-kit.htm http://www.umbrellalifescience.co.in/soil-dna-isolation-kit.htm Mon, 17 Jun 2019 00:00:00 +0530 Features Soil DNA Isolation Kit provides a high quality, convenient and easy-to use method technique to isolate high quality DNA from soil samples. The kit uses Fast-Spin column technology based in DNA ability to bind silica in the presence of high concentrations of chaotropic salts as guanidinium thiocyanate. Extractions are rapidly and efficiently lysed by bead beating. The sample DNA is then bound to the surface of a silica membrane.   Advantages & Features : High quality DNA isolated from soil samples. Time-Saving : rapid isolation of ready-to-use DNA within 58 minutes. Easy, safe and convenient : avoids phenol/chloroform extraction. High purity : eliminate humic acid, polysaccharides, phenol compounds and enzyme inhibitor from stool sample. Sample Size : mini Kit Prep: 0.2~1g of soil sample. Efficient : soil samples are lysed by bead beating    Applications: All Molecular Biology applications, such as: Digestion with restriction enzymes. Automated sequencing. PCR template. Southern Blots.    Quality Control: Tested for isolation of DNA from soil sample. The quantity and quality of purified DNA attend to: Ratio 260/280 (1.8-2.0). Agarose gel electrophoresis FFPE DNA Isolation Kit http://www.umbrellalifescience.co.in/ffpe-dna-isolation-kit.htm http://www.umbrellalifescience.co.in/ffpe-dna-isolation-kit.htm Mon, 17 Jun 2019 00:00:00 +0530 Features Formalin fixation and paraffin embedding (FFPE) DNA Isolation Kit is an easy, rapid and efficient method for long-term preservation of most archived pathological specimens. It extraction involves two different phases: Deparaffinization and DNA extraction. Fast-Spin column technology omits the use of flammable and malodorous xylene or d-limonene commonly used for deparaffinization, a proprietary buffer formulation Deparaffinization solution is used for the complete dissolution of the wax to release the tissue.   Advantages & Features : High quality : of DNA obtained. Very easy procedure : it takes 173 minutes to paraffin removal with minimal handling. Safe : avoids xylene and other toxic solvents. High durability : optimized for long-term preservation of most archived pathological specimens. Complete solution : includes two different phases Deparaffinization and DNA extraction.    Applications : Rapid isolation of DNA from formalin-fixed, paraffin-embedded samples. Isolation of DNA from fresh and archived FFPE samples. Isolation of DNA from specimen of object slides. Typical downstream applications: PCR, qPCR, NGS, STR analysis.    Quality Control : DNA isolated from tissue slice paraffin-embedded (10 μm) quantified by spectrophotometer : Ratio 260/280 (1.8-2.0). Agarose gel electrophoresis Stool DNA Isolation Kit http://www.umbrellalifescience.co.in/stool-dna-isolation-kit.htm http://www.umbrellalifescience.co.in/stool-dna-isolation-kit.htm Mon, 17 Jun 2019 00:00:00 +0530 Features The DNA Stool Mini Kit provides fast and easy purification of total DNA from fresh or frozen stool samples. Purified DNA is of high quality and well suited for use in PCR and other downstream enzymatic reactions. The simple spin procedure yields pure DNA ready for direct use in less than 1 hour.   Advantages & Features: High quality DNA isolated from stool samples. Time-Saving : rapid isolation of ready-to-use DNA within 58 minutes. Easy, safe and convenient : avoids phenol/chloroform extraction. High purity : Eliminate humic acid, polysaccharides, phenol compounds and enzyme inhibitor from stool sample.    Applications: All Molecular Biology applications, such as : Digestion with restriction enzymes. Automated sequencing. PCR template. Southern Blots.    Quality Control : The quantity and quality of purified DNA attend to: Ratio 260/280 (1.8-2.0). Agarose gel electrophoresis DNA Gel Purification Kit http://www.umbrellalifescience.co.in/dna-gel-purification-kit.htm http://www.umbrellalifescience.co.in/dna-gel-purification-kit.htm Mon, 17 Jun 2019 00:00:00 +0530 Features Agarose Purification Kit provides an accurate, rapid and efficient method to extract DNA from agarose gels. The kit uses Fast-Spin column technology based in solubilisation and binding of DNA to a silica membrane in presence of chaotropic salts. Comfortable MiniSpin Columns contains an exclusive membrane that allows binding a unique DNA fragment, previously excised from agarose gel.   Advantages & Features : Extremely fast and easy procedure : it takes 5 minutes to results with minimal handling steps. Reproducible yields : > 80% DNA Recovery (0.7 -1% agarose) and reproducible yields of pure DNA. Versatile : compatible with a wide spectrum of size fragments, suitable since 100 bp up or any kind of agarose and gel buffer systems. Pure genomic DNA : ready-to-use in all Molecular Biology applications.    Applications : Purification of DNA fragments (obtained by PCR or digestion with restriction enzymes) from agarose gels. Purified DNA suitable for all common Molecular Biology applications, such as:    Quality Control : The quantity and quality of purified DNA attend to: Ratio 260/280 (1.8-2.0). Agarose gel electrophoresis PCR Purification Kit http://www.umbrellalifescience.co.in/pcr-purification-kit.htm http://www.umbrellalifescience.co.in/pcr-purification-kit.htm Mon, 17 Jun 2019 00:00:00 +0530 Features PCR Purification kit provides an efficient, consistent and rapid method to purify DNA and remove contaminants from reaction mixtures (e.g. PCR, digestion or labeling reactions). Comfortable MiniSpin Columns contains an exclusive membrane that allows DNA adsorption in presence of chaotropic salts and the removal of contaminants.   Advantages & Features : Really fast and easy procedure : it takes 14 minutes to results with minimal handling steps. Reproducible yields : high DNA Recovery (70-90%) and consistent yields of pure DNA. Sensitive : proven performance for DNA fragments as short as 75 bp. Pure genomic DNA : ready-to-use in all Molecular Biology applications.    Applications : Removal of proteins and salts from PCR, restriction digestion, dephosphorylation, ligation or labelling reactions. Changing of a restriction enzyme buffer. Re-purification of genomic DNA.    Quality Control : Tested in the purification of a 0.6 kb DNA fragment from PCR mixture. Purified band is analysed in agarose gel electrophoresis. Saliva Collection & DNA Isolation Kit http://www.umbrellalifescience.co.in/saliva-collection-dna-isolation-kit.htm http://www.umbrellalifescience.co.in/saliva-collection-dna-isolation-kit.htm Mon, 17 Jun 2019 00:00:00 +0530 Saliva Collection Device http://www.umbrellalifescience.co.in/saliva-collection-device.htm http://www.umbrellalifescience.co.in/saliva-collection-device.htm Mon, 17 Jun 2019 00:00:00 +0530 RNA Isolation Kit http://www.umbrellalifescience.co.in/rna-isolation-kit.htm http://www.umbrellalifescience.co.in/rna-isolation-kit.htm Mon, 17 Jun 2019 00:00:00 +0530 Bacterial RNA Isolation Kit http://www.umbrellalifescience.co.in/bacterial-rna-isolation-kit.htm http://www.umbrellalifescience.co.in/bacterial-rna-isolation-kit.htm Mon, 17 Jun 2019 00:00:00 +0530 Features Bacterial Isolation Mini Kit is designed for the rapid preparation of RNA from a wide varieties of bacterial species. Purification is based on spin column chromatography as the separation matrix. This kit is optimized for RNA purification from both Gram-negative and Gram-positive bacterial cells. The lysate is passed through DNA clearance column which will eliminate DNA.   Advantages & Features : Highly efficient : yields up to 24 μg. Really fast and easy procedure : it takes 25 minutes to get results with minimal handling steps. Safe : avoids phenol/chloroform extraction.    Applications : Purified RNA suitable for all common Molecular Biology applications, such as: RT-PCR. Northern blotting    Quality Control : Tested on a lot-to-lot basis by isolating total RNA from different bacteria. The quantity and quality of purified RNA attend to: Ratio 260/280 (1.8-2.0). Agarose gel electrophoresis. Plant RNA Isolation kit http://www.umbrellalifescience.co.in/plant-rna-isolation-kit.htm http://www.umbrellalifescience.co.in/plant-rna-isolation-kit.htm Mon, 17 Jun 2019 00:00:00 +0530 Features Plant RNA Purification Kit offers a consistent, easy-to use and rapid method for purification of Total RNA from a variety of plant tissue. The kit uses Fast-Spin column technology based in RNA ability to bind silica in the presence of high concentrations of chaotropic salts.   Advantages & Features : Highly efficient : yields up to ˞30 μg Total RNA from young leaves. Pure RNA : ready-to-use in all Molecular Biology applications. Easy and fast procedure : it takes 28 minutes with minimal handling. Safe : avoids phenol-chloroform extraction, centrifugation with CsCl gradients or precipitation with LiCl. Mini format.    Applications : Purified RNA suitable for all common Molecular Biology applications, such as: RT-PCR. Northern blotting    Quality Control : Total RNA is isolated from a 100 mg young leaf sample. Purified RNA is quantified using a spectrophotometer with a typical yield of more than 10 μg of Total RNA and an A260nm/A280nm ratio of 1.9-2.1. Quality is further checked by agarose gel electrophoresis. Tissue RNA Isolation Kit http://www.umbrellalifescience.co.in/tissue-rna-isolation-kit.htm http://www.umbrellalifescience.co.in/tissue-rna-isolation-kit.htm Mon, 17 Jun 2019 00:00:00 +0530 Features Tissue Total RNA Purification Kit offers an efficient, rapid and convenient method for purification of Total RNA from a variety of tissue and culture cells. The kit is based in nucleic acid ability to bind silica in the presence of high concentrations of chaotropic salts. Tissue samples can be efficiently homogenized in a microcentrifuge tube using the provided micropestle.   Advantages & Features : Highly efficient : yields up to to 50 μg, depends on type of sample. Pure RNA : ready-to-use in all Molecular Biology applications. Easy and fast procedure : it takes 28 minutes with minimal handling. Mini format.    Applications : Total RNA is isolated from a 30 mg thorax muscle tissue sample. Purified RNA is quantified using a spectrophotometer with a typical yield of more than 10 μg of Total RNA and an A260nm/A280nm ratio of 1.9-2.1. Quality is further checked by agarose gel electrophoresis. Highly efficient: yields up to to 50 μg, depends on type of sample. Pure RNA: ready-to-use in all Molecular Biology applications. Easy and fast procedure: it takes 28 minutes with minimal handling. Mini form Viral DNA & RNA Isolation Kit http://www.umbrellalifescience.co.in/viral-dna-rna-isolation-kit.htm http://www.umbrellalifescience.co.in/viral-dna-rna-isolation-kit.htm Mon, 17 Jun 2019 00:00:00 +0530 Features Viral DNA/RNA Mini Spin Kit offers an efficient, fast and simultaneous purification of viral DNA and RNA from cell–free samples such as serum, plasma and cerebrospinal fluid. The kit uses Fast-Spin column technology based in nucleic acid ability to bind silica in the presence of high concentrations of chaotropic salts. The viral RNA/DNA molecules bind to the silica-based media and impurities such as proteins and nucleases are removed by thorough washing with Wash Buffer. The RNA/DNA is then eluted in sterile, Rnase free water.   Advantages & Features : Efficient : 3-6 μg of genomic DNA from a 200 μl plasma/serum. Fast : results in less of 28 minutes. Safe : no phenol-chloroform extraction. Ready-to-use : genomic DNA, in all Molecular Biology applications    Applications : Purified DNA suitable for all common Molecular Biology applications, such as: RT-PCR. qRT-PCR. qPCR. Viral load monitoring. Viral detection. Viral genotyping.    Quality Control : DNA isolated from 200 μl of plasma/serum and analysed by PCR. RNA Isolater Total RNA Extraction Reagent http://www.umbrellalifescience.co.in/rna-isolater-total-rna-extraction-reagent.htm http://www.umbrellalifescience.co.in/rna-isolater-total-rna-extraction-reagent.htm Mon, 17 Jun 2019 00:00:00 +0530 Features RNA Isolater Total RNA Extraction Reagent uses guanidinium isothiocyanate and phenol, with high lysis ability. It can lyse cells and tissues in short time, keeping integrity of RNA. The RNA isolater Total RNA Extraction Reagent is widely used for RNA extraction of cultured cells, animal tissues, microorganisms, and plant tissues with weak secondary metabolism (i.e. seedling and spire). Samples will be lysed thoroughly in RNA isolater and form supernatant, interlayer, and organic layer (bright red sublayer). RNA is distributed in the upper water phase. Remove the RNA-containing supernatant layer and treat with isopropanol to extract the total RNA.   Tissue & Cultured cells ↓ Homogenize in RNA isolater or triturate in liquid nitrogen and dissolve by RNA isolater. ↓ Add Chloroform of 1/5 solution volume, mix vigorously, and place on ice (4℃) for 5 min. ↓ Centrifuge at 12,000 × g for 15 min at 4℃. ↓ Transfer the supernatant to a new tube, add equal volume of isopropanol, and place on ice (4℃) for 10 min. ↓ Centrifuge at 12,000 × g for 10 min at 4℃. ↓ Rinse with 75% ethanol. ↓ Centrifuge at 12,000 × g for 5 min at 4℃. ↓ Discard the supernatant, dry the RNA-containing pellet, and dissolve the pellet with DEPC treated water. ↓ Test the integrity of RNA using gel electrophoresis, test the purity and concentration with a spectrophotometer. DNA & RNA Isolation Kit http://www.umbrellalifescience.co.in/dna-rna-isolation-kit.htm http://www.umbrellalifescience.co.in/dna-rna-isolation-kit.htm Mon, 17 Jun 2019 00:00:00 +0530 TRIzol Plus RNA Purification Kit http://www.umbrellalifescience.co.in/trizol-plus-rna-purification-kit.htm http://www.umbrellalifescience.co.in/trizol-plus-rna-purification-kit.htm Mon, 17 Jun 2019 00:00:00 +0530 PCR Reagents http://www.umbrellalifescience.co.in/pcr-reagents.htm http://www.umbrellalifescience.co.in/pcr-reagents.htm Mon, 17 Jun 2019 00:00:00 +0530 RT-PCR Reagents http://www.umbrellalifescience.co.in/rt-pcr-reagents.htm http://www.umbrellalifescience.co.in/rt-pcr-reagents.htm Mon, 17 Jun 2019 00:00:00 +0530 M-MLV Reverse Transcriptase http://www.umbrellalifescience.co.in/m-mlv-reverse-transcriptase.htm http://www.umbrellalifescience.co.in/m-mlv-reverse-transcriptase.htm Mon, 17 Jun 2019 00:00:00 +0530 Features MMLV Reverse Transcriptase (MMLV-RT), encoded by Moloney Murine Leukemia Virus (MMLV) is an RNA-dependent DNA polymerase that synthesizes the cDNA first strand from a single-stranded RNA template to which a primer has been hybridized. MMLV-RT will also extend primers hybridized tosingle-stranded DNA.   Advantages & Features : RT PCR. Synthesis of cDNA. mRNA 5’-end Mapping by Primer Extension Analysis. End-labeling of DNA. Dideoxynucleotide Sequencing.    Quality Control : Exempt of nucleases (endo, exo and ribonucleases)activities. Purity: >90% as judged by SDS-polyacrylamide gels with blue staining. 1st Strand cDNA Synthesis Kit http://www.umbrellalifescience.co.in/1st-strand-cdna-synthesis-kit.htm http://www.umbrellalifescience.co.in/1st-strand-cdna-synthesis-kit.htm Mon, 17 Jun 2019 00:00:00 +0530 Features 1st Strand cDNA Synthesis Kit provides all the necessary components for cDNA synthesis from total RNA or mRNA. The cDNA is efficiently synthesized by TRUEScript Hˉ RTase /RI Mix and 5X RT Reaction Mix(dNTP included ). Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis. cDNA up to 12 kb.   Advantages & Features : Highest purity : > 99% confirmed by HPLC. Highly stable. Proven performance for PCR. High Quality : free of DNAse, protease, phosphatase, nuclease, Human or E. coli DNA contamination.    Applications :  For use in all common Molecular Biology applications, such as: PCR. Real-time PCR. High fidelity and long PCR. ·RT-PCR. cDNA synthesis. ·DNA sequencing. ·DNA amplification One Step RT-PCR Kit http://www.umbrellalifescience.co.in/one-step-rt-pcr-kit.htm http://www.umbrellalifescience.co.in/one-step-rt-pcr-kit.htm Mon, 17 Jun 2019 00:00:00 +0530 Features One-Step RT-PCR Kit is designed for the reverse transcription (RT) and polymerase chain reaction (PCR) amplification of a specific target RNA from either total RNA or mRNA. This system uses a mixture of MMLV Reverse Transcriptase and Hot start Taq DNA polymerase in an optimized reaction buffer, and can detect RNA targets up to 4.0 kb. The amount of input total RNA can range from 0.01 pg to 2 μg. Sufficient reagents are provided for 25 or 100 amplification reactions of 50 μl each.   Advantages & Features : Fast and easy one-tube setup Efficient one-step RT-PCR of any RNA template without optimization Unique enzyme mix for high specificity and sensitivity Balanced mixture of enzymes with optimized reverse-transcription buffer    Applications :  For use in all common Molecular Biology applications, such as : PCR. Real-time PCR. High fidelity and long PCR. RT-PCR. cDNA synthesis. DNA sequencing. DNA amplification Hot Start PCR Master Mix http://www.umbrellalifescience.co.in/hot-start-pcr-master-mix.htm http://www.umbrellalifescience.co.in/hot-start-pcr-master-mix.htm Mon, 17 Jun 2019 00:00:00 +0530 SYBR Green qPCR Mix http://www.umbrellalifescience.co.in/sybr-green-qpcr-mix.htm http://www.umbrellalifescience.co.in/sybr-green-qpcr-mix.htm Mon, 17 Jun 2019 00:00:00 +0530 Features 2 x SYBR Green qPCR Mix provides rapid real-time quantification of RNA targets in SYBR Green fluorescent. The components of 2 x SYBR Green qPCR Mix include Hot-start enzyme named HotMaster Taq DNA Polymerase, dNTP, PCR reaction buffer, BSA and SYBR Green , and so on. The fluorescent dye SYBR Green I in the master mix enables the analysis of many different targets without having to synthesize target specific labeled probes.   Advantages & Features : Nucleic acid amplification and expression profiling Gene expression analysis Genomics-related applications Genotyping Mutation detection Pathogen detection    SYBR Green Master Mix Components SYBR Green master mixes are 2x concentrated, and contain dNTPs, MgCl2, and DNA polymerase. SYBR Green master mixes are available with other dyes including ROX. Many PCR machines require a passive reference dye. An internal reference dye, such as ROX, corrects well-to-well optical variations, and is used for fluorescent signal normalization. SYBR Green qRT -PCR Kit http://www.umbrellalifescience.co.in/sybr-green-qrt-pcr-kit.htm http://www.umbrellalifescience.co.in/sybr-green-qrt-pcr-kit.htm Mon, 17 Jun 2019 00:00:00 +0530 Features The SYBR Green RT-PCR Kit provides highly specific quantification of RNA targets by real-time one-step RT-PCR using SYBR Green I detection. The combination of a hot start and a unique qRT-PCR buffer system ensures highly specific and sensitive real-time quantification of RNA targets. The dNTP mix includes dUTP, allowing optional treatment with UNG. The SYBR Green RT-PCR Kit is also supplied with an optimized RT mix for efficient and sensitive reverse transcription over a wide range of RNA template amounts. For convenience, the master mix can be stored at 2–8°C.   Advantages & Features: Fast and easy one-tube setup Efficient one-step RT-PCR of any RNA template without optimization Unique enzyme mix for high specificity and sensitivity Balanced mixture of enzymes with optimized reverse-transcription buffer    Applications : For use in all common Molecular Biology applications, such as: PCR. Real-time PCR. High fidelity and long PCR. RT-PCR. cDNA synthesis. DNA sequencing. DNA amplification DNA Ladders http://www.umbrellalifescience.co.in/dna-ladders.htm http://www.umbrellalifescience.co.in/dna-ladders.htm Mon, 17 Jun 2019 00:00:00 +0530 100 bp DNA Ladder http://www.umbrellalifescience.co.in/100-bp-dna-ladder.htm http://www.umbrellalifescience.co.in/100-bp-dna-ladder.htm Mon, 17 Jun 2019 00:00:00 +0530 Features Highly efficient, bright and accurate DNA Ladders for visualization on agarose gel DNA size standards. Umbrella Life Science manufactures 4 different DNA size standards for small and large DNA molecules. These standards are ready-to-use markers which contain loading dye with one or two migration visualisation dye.   Advantages & Features : Ready-to-use format to perfect visualization on agarose gel. Well-defined bands patterns. Highly Stable at Room Temperature. Bright and accurate. Tested with Ethidium bromide and Gel green.    Applications : Molecular weight standards for gel electrophoresis 1 kb DNA Ladder http://www.umbrellalifescience.co.in/1-kb-dna-ladder.htm http://www.umbrellalifescience.co.in/1-kb-dna-ladder.htm Mon, 17 Jun 2019 00:00:00 +0530 Features Highly efficient, bright and accurate DNA Ladders for visualization on agarose gel DNA size standards. Umbrella Life Science manufactures 4 different DNA size standards for small and large DNA molecules. These standards are ready-to-use markers which contain loading dye with one or two migration visualisation dye.   Advantages & Features : Ready-to-use format to perfect visualization on agarose gel. Well-defined bands patterns. Highly Stable at Room Temperature. Bright and accurate. Tested with Ethidium bromide and Gel green.    Applications : Molecular weight standards for gel electrophoresis 1 kb DNA Plus Ladder http://www.umbrellalifescience.co.in/1-kb-dna-plus-ladder.htm http://www.umbrellalifescience.co.in/1-kb-dna-plus-ladder.htm Mon, 17 Jun 2019 00:00:00 +0530 Features Highly efficient, bright and accurate DNA Ladders for visualization on agarose gel DNA size standards. Umbrella Life Science manufactures 4 different DNA size standards for small and large DNA molecules. These standards are ready-to-use markers which contain loading dye with one or two migration visualisation dye.   Advantages & Features : Ready-to-use format to perfect visualization on agarose gel. Well-defined bands patterns. Highly Stable at Room Temperature. Bright and accurate. Tested with Ethidium bromide and Gel green.    Applications : Molecular weight standards for gel electrophoresis Hind III DNA Ladder http://www.umbrellalifescience.co.in/hind-iii-dna-ladder.htm http://www.umbrellalifescience.co.in/hind-iii-dna-ladder.htm Mon, 17 Jun 2019 00:00:00 +0530 Features Highly efficient, bright and accurate DNA Ladders for visualization on agarose gel DNA size standards. Umbrella Life Science manufactures 4 different DNA size standards for small and large DNA molecules. These standards are ready-to-use markers which contain loading dye with one or two migration visualisation dye.   Advantages & Features : Ready-to-use format to perfect visualization on agarose gel. Well-defined bands patterns. Highly Stable at Room Temperature. Bright and accurate. Tested with Ethidium bromide and Gel green.    Applications : Molecular weight standards for gel electrophoresis Nucleic Acid Modifying Enzymes http://www.umbrellalifescience.co.in/nucleic-acid-modifying-enzymes.htm http://www.umbrellalifescience.co.in/nucleic-acid-modifying-enzymes.htm Mon, 17 Jun 2019 00:00:00 +0530 Electrophoresis http://www.umbrellalifescience.co.in/electrophoresis.htm http://www.umbrellalifescience.co.in/electrophoresis.htm Mon, 17 Jun 2019 00:00:00 +0530 100mg Molecular Biology Grade Agarose http://www.umbrellalifescience.co.in/100mg-molecular-biology-grade-agarose.htm http://www.umbrellalifescience.co.in/100mg-molecular-biology-grade-agarose.htm Mon, 17 Jun 2019 00:00:00 +0530 Features Agarose is a standard melting temperature, multi-purpose agarose that is ideal for routine separation analysis. Agarose resolves DNA and RNA fragments from 500–23,000 bp, and has no detectable DNase or RNase activity.   Advantages & Features : Appearance : White homegeous powder Gel strength of 1.5 % (w/v) gel : >1680g / cm2 Gel strength of 3 % (w/v) gel : >3290g / cm2 Gelling temperature :33-34°C Melting temperature : 74°C  EEO : 0.1-0.2 500mg Molecular Biology Grade Agarose http://www.umbrellalifescience.co.in/500mg-molecular-biology-grade-agarose.htm http://www.umbrellalifescience.co.in/500mg-molecular-biology-grade-agarose.htm Mon, 17 Jun 2019 00:00:00 +0530 Features Agarose is a standard melting temperature, multi-purpose agarose that is ideal for routine separation analysis. Agarose resolves DNA and RNA fragments from 500–23,000 bp, and has no detectable DNase or RNase activity.   Advantages & Features : Appearance : White homegeous powder Gel strength of 1.5 % (w/v) gel : >1680g / cm2 Gel strength of 3 % (w/v) gel : >3290g / cm2 Gelling temperature :33-34°C Melting temperature : 74°C  EEO : 0.1-0.2 RNA Loading Dye http://www.umbrellalifescience.co.in/rna-loading-dye.htm http://www.umbrellalifescience.co.in/rna-loading-dye.htm Mon, 17 Jun 2019 00:00:00 +0530 Features 2X RNA Loading Dye is recommended for preparation of RNA samples for electrophoresis on agarose or polyacrylamide gels. It contains the tracking dyes bromophenol blue and xylene cyanol FF as well as the intercalating dye ethidium bromide.   Advantages & Features : Two-color tracking of DNA, RNA migration during electrophoresis No DNA, RNA masking during gel exposure to UV light Denaturation of DNA, RNA due to the presence of formamide DNA Loading Dye http://www.umbrellalifescience.co.in/dna-loading-dye.htm http://www.umbrellalifescience.co.in/dna-loading-dye.htm Mon, 17 Jun 2019 00:00:00 +0530 Features 6X BX/Loading Buffer is used as a loading dye for visual tracking of DNA migration during electrophoresis. It incorporates Bromophenol blue and Xylene Cyanol FF as tracking dye. Bromophenol blue migrates fast in the agarose gel and corresponds to the migration of a 300 - 500 bp long DNA fragment in a 1% agarose gel. Xylene cyanol FF migrates comparatively slower and corresponds to the migration of a 4,000 –5,000 bp long DNA fragment in a 1% agarose gel.   Advantages & Features : Two-colour tracking of DNA migration during DNA electrophoresis No DNA masking during gel exposure to UV light EDTA binds divalent metal ions and inhibits metal dependent nucleases    Applications : Preparation of DNA ladders, markers, and samples for loading on agarose or polyacrylamide gels RNA Stabilization Solution http://www.umbrellalifescience.co.in/rna-stabilization-solution.htm http://www.umbrellalifescience.co.in/rna-stabilization-solution.htm Mon, 17 Jun 2019 00:00:00 +0530 Features RNA Solution makes it possible for researchers to postpone RNA isolation for days, weeks, or even months after tissue collection without sacrificing RNA integrity. All we need to do is to add 10 times volume of RNA Solution into the tube containing the freshly collected tissue (1 ml RNA Solution to 100 mg tissue) and store the tube at –20℃ until use   Advantages & Features: Useful in molecular biology processes Perfect for tissue collection "in the field“ Flexible tissue storage -RNA is stable for 1 day at 37°C, 1 week at 25°C, 1 month at 4°C or long term at -20°C Compatible with many RNA isolation procedures Nucleic Acid Isolation Columns http://www.umbrellalifescience.co.in/nucleic-acid-isolation-columns.htm http://www.umbrellalifescience.co.in/nucleic-acid-isolation-columns.htm Mon, 17 Jun 2019 00:00:00 +0530 PCR Kit http://www.umbrellalifescience.co.in/pcr-kit.htm http://www.umbrellalifescience.co.in/pcr-kit.htm Mon, 17 Jun 2019 00:00:00 +0530 Plant Direct PCR Kit http://www.umbrellalifescience.co.in/plant-direct-pcr-kit.htm http://www.umbrellalifescience.co.in/plant-direct-pcr-kit.htm Mon, 17 Jun 2019 00:00:00 +0530 Tissue Direct PCR Kit http://www.umbrellalifescience.co.in/tissue-direct-pcr-kit.htm http://www.umbrellalifescience.co.in/tissue-direct-pcr-kit.htm Mon, 17 Jun 2019 00:00:00 +0530